Exploring modulation of action potential firing by artificial graft of fast GABAergic autaptic afferences in hypophyseal neuroendocrine melanotrope cells.
Identifieur interne : 003329 ( Main/Exploration ); précédent : 003328; suivant : 003330Exploring modulation of action potential firing by artificial graft of fast GABAergic autaptic afferences in hypophyseal neuroendocrine melanotrope cells.
Auteurs : Sofiane Boussa [France] ; Jennifer Pasquier ; François Leboulenger ; Alain Faure ; Frank Le FollSource :
- Journal of physiology, Paris [ 1769-7115 ]
English descriptors
- KwdEn :
- Action Potentials (drug effects), Action Potentials (physiology), Animals, Biophysics, Cells, Cultured, Electric Stimulation (methods), Feedback, Physiological (drug effects), Feedback, Physiological (physiology), Male, Melanotrophs (drug effects), Melanotrophs (physiology), Models, Neurological, Neural Inhibition (drug effects), Neural Inhibition (physiology), Patch-Clamp Techniques (methods), Pituitary Gland (cytology), Rana ridibunda, Time Factors, gamma-Aminobutyric Acid (metabolism), gamma-Aminobutyric Acid (pharmacology).
- MESH :
- chemical , metabolism : gamma-Aminobutyric Acid.
- cytology : Pituitary Gland.
- drug effects : Action Potentials, Feedback, Physiological, Melanotrophs, Neural Inhibition.
- methods : Electric Stimulation, Patch-Clamp Techniques.
- chemical , pharmacology : gamma-Aminobutyric Acid.
- physiology : Action Potentials, Feedback, Physiological, Melanotrophs, Neural Inhibition.
- Animals, Biophysics, Cells, Cultured, Male, Models, Neurological, Rana ridibunda, Time Factors.
Abstract
Excitable cells are connected via electrical and chemical synapses to form a complex plastic network that transmit and modify action potential trains. In vivo and in vitro studies suggest that powerful type A GABAergic self-innervations, known as autapses, play a central role in the shaping of spike discharges. Herein, we have investigated the effects of artificial autaptic activity on action potential firing in cultured hypophyseal neuroendocrine melanotrope cells removed from any synaptic input. Type A autaptic conductances were introduced by using a dedicated dynamic-clamp device, based on a digital signal processor. The results indicate that cells grafted with autaptic dynamic-clamp are subjected to a modulation of both evoked firing and artificial GABA(A)-currents. The autaptic feedback reduced the action potentials amplitude, decreasing both the overshoot and the after-hyperpolarization potential (AHP). In return, the reduced voltage changes diminished the autaptic current amplitudes. The overall effect was a decrease of the cell firing rate. The introduction of a variable autaptic delay strongly altered the cell responses. Under certain conditions, the artificial autaptic current formed an additional component of the AHP which was markedly augmented. This action resulted in a stabilization of the action potential train leading to a more regular firing. In conclusion, it appeared that the autaptic feedback was very dependent on functional parameters such as the autaptic distance. Further investigations are in progress to complete our model, taking the autaptic plasticity into account.
DOI: 10.1016/j.jphysparis.2009.11.008
PubMed: 19909812
Affiliations:
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first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Faure, Alain" sort="Faure, Alain" uniqKey="Faure A" first="Alain" last="Faure">Alain Faure</name></author><author><name sortKey="Foll, Frank Le" sort="Foll, Frank Le" uniqKey="Foll F" first="Frank Le" last="Foll">Frank Le Foll</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2010 Jan-Mar</MedlineDate></PubDate></date><idno type="RBID">pubmed:19909812</idno><idno type="pmid">19909812</idno><idno type="doi">10.1016/j.jphysparis.2009.11.008</idno><idno type="wicri:Area/PubMed/Corpus">000237</idno><idno type="wicri:Area/PubMed/Curation">000237</idno><idno type="wicri:Area/PubMed/Checkpoint">000237</idno><idno type="wicri:Area/Ncbi/Merge">000240</idno><idno type="wicri:Area/Ncbi/Curation">000240</idno><idno type="wicri:Area/Ncbi/Checkpoint">000240</idno><idno type="wicri:Area/Main/Merge">003416</idno><idno type="wicri:Area/Main/Curation">003329</idno><idno type="wicri:Area/Main/Exploration">003329</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Exploring modulation of action potential firing by artificial graft of fast GABAergic autaptic afferences in hypophyseal neuroendocrine melanotrope cells.</title><author><name sortKey="Boussa, Sofiane" sort="Boussa, Sofiane" uniqKey="Boussa S" first="Sofiane" last="Boussa">Sofiane Boussa</name><affiliation wicri:level="1"><nlm:affiliation>UPRES-EA, University of Le Havre, France. sofiane.boussa@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UPRES-EA, University of Le Havre</wicri:regionArea><wicri:noRegion>University of Le Havre</wicri:noRegion><wicri:noRegion>University of Le Havre</wicri:noRegion></affiliation></author><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Faure, Alain" sort="Faure, Alain" uniqKey="Faure A" first="Alain" last="Faure">Alain Faure</name></author><author><name sortKey="Foll, Frank Le" sort="Foll, Frank Le" uniqKey="Foll F" first="Frank Le" last="Foll">Frank Le Foll</name></author></analytic><series><title level="j">Journal of physiology, Paris</title><idno type="eISSN">1769-7115</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Action Potentials (drug effects)</term><term>Action Potentials (physiology)</term><term>Animals</term><term>Biophysics</term><term>Cells, Cultured</term><term>Electric Stimulation (methods)</term><term>Feedback, Physiological (drug effects)</term><term>Feedback, Physiological (physiology)</term><term>Male</term><term>Melanotrophs (drug effects)</term><term>Melanotrophs (physiology)</term><term>Models, Neurological</term><term>Neural Inhibition (drug effects)</term><term>Neural Inhibition (physiology)</term><term>Patch-Clamp Techniques (methods)</term><term>Pituitary Gland (cytology)</term><term>Rana ridibunda</term><term>Time Factors</term><term>gamma-Aminobutyric Acid (metabolism)</term><term>gamma-Aminobutyric Acid (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>gamma-Aminobutyric Acid</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Pituitary Gland</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Action Potentials</term><term>Feedback, Physiological</term><term>Melanotrophs</term><term>Neural Inhibition</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electric Stimulation</term><term>Patch-Clamp Techniques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>gamma-Aminobutyric Acid</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Action Potentials</term><term>Feedback, Physiological</term><term>Melanotrophs</term><term>Neural Inhibition</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biophysics</term><term>Cells, Cultured</term><term>Male</term><term>Models, Neurological</term><term>Rana ridibunda</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Excitable cells are connected via electrical and chemical synapses to form a complex plastic network that transmit and modify action potential trains. In vivo and in vitro studies suggest that powerful type A GABAergic self-innervations, known as autapses, play a central role in the shaping of spike discharges. Herein, we have investigated the effects of artificial autaptic activity on action potential firing in cultured hypophyseal neuroendocrine melanotrope cells removed from any synaptic input. Type A autaptic conductances were introduced by using a dedicated dynamic-clamp device, based on a digital signal processor. The results indicate that cells grafted with autaptic dynamic-clamp are subjected to a modulation of both evoked firing and artificial GABA(A)-currents. The autaptic feedback reduced the action potentials amplitude, decreasing both the overshoot and the after-hyperpolarization potential (AHP). In return, the reduced voltage changes diminished the autaptic current amplitudes. The overall effect was a decrease of the cell firing rate. The introduction of a variable autaptic delay strongly altered the cell responses. Under certain conditions, the artificial autaptic current formed an additional component of the AHP which was markedly augmented. This action resulted in a stabilization of the action potential train leading to a more regular firing. In conclusion, it appeared that the autaptic feedback was very dependent on functional parameters such as the autaptic distance. Further investigations are in progress to complete our model, taking the autaptic plasticity into account.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Faure, Alain" sort="Faure, Alain" uniqKey="Faure A" first="Alain" last="Faure">Alain Faure</name><name sortKey="Foll, Frank Le" sort="Foll, Frank Le" uniqKey="Foll F" first="Frank Le" last="Foll">Frank Le Foll</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></noCountry><country name="France"><noRegion><name sortKey="Boussa, Sofiane" sort="Boussa, Sofiane" uniqKey="Boussa S" first="Sofiane" last="Boussa">Sofiane Boussa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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